Solid-Phase Tryptophan Synthase Biocatalysts and Their Application in Integrated Bioconversion System

Abstract

Setting-up possibilities of an integrated system consisting of the tryptophan biosynthesis step and of a product separation step have been studied. The aim was to increase the productivity of the bioprocess through reducing the effect of product inhibition. Adsorption of the product onto XAD-4 neutral resin has proved to be selective enough for its separation from the substrates. The system with alternate operation of the catalytic bioconversion step and the product adsorption step seems to be applicable for production of tryptophan but enhancement of the working stability needs further investigations.