Production of Short Chain Fructooligosaccharides

Abstract

Pectinex Ultra SP-L, a commercial enzyme preparation with fructosyl-transferase activity and ability to produce short chain fructooligosaccharides, was immobilized onto anionic ionexchange resin by a combined method. During the work this solid-phase biocatalyst was used for the production of fructooligosaccharides from saccharose, where glucose was formed as inhibiting by-product. In the experiments the optimal biocatalyst/matrix ratio and the optimal immobilization conditions: concentration of cross-linking agent, immobilization time and optimal operational conditions: temperature and pH were determined. Moreover an integrated reactor system was constructed for simultaneous fructooligosaccharides production synthesis and glucose elimination to enhance the product yield.