A NOVEL METHOD FOR ISOLATION OF LIPOXYGENASE-1 ISOENZYME FROM SOYBEAN MEAL

Authors

  • Á. S. Németh
  • B. Szajáni
  • J. S. Márczy
  • M. L. Simon

DOI:

https://doi.org/10.1515/hjic-2001-07

Keywords:

soybean, Glycine max, lipoxygenase isoenzymes, selective heat treatment

Abstract

A novel method has been elaborated for the isolation of lipoxygenase-1 (Lox-1) isoenzyme from soybean meal. The essential step of the procedure is the selective heat treatment of soybean meal extract at 70 °C in a medium of 0.05 ionic strength and pH 5.2 for 5 min. The heat treated extract practically free from lipoxygenase-2 (Lox-2) and lipoxygenase-3 (Lox-3) isoenzymes was purified by DEAE-cellulose ion exchange chromatography. The resulted lipoxygenase-1 isoenzyme was homogeneous as judged by PAGE. A tenfold purification and a relatively high yield (39%) was achieved.

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Published

2001-10-12

Issue

Vol 29, No 1 (2001): Hungarian Journal of Industrial Chemistry

Section

Articles

How to Cite

A NOVEL METHOD FOR ISOLATION OF LIPOXYGENASE-1 ISOENZYME FROM SOYBEAN MEAL. (2001). Hungarian Journal of Industry and Chemistry, 29(1), 35-38. https://doi.org/10.1515/hjic-2001-07