Examination of Crystallization Coupled to the SMB-LC Process

Abstract

The simulated moving bed preparative liquid chromatography (SMB-LC) technique is a highly promising method for optical isomer separation. Optimal coupling of SMB-LC to crystallization respectfully increases the effect of enantioseparation. Extract and raffinate products enriched by SMB-LC are suitable for further process by evaporation, cooling crystallization. Chromatographic packing and eluent system selection being the best for the separation and determination of optimal working parameters of SMB-LC coupled with crystallization can be found in our previous publications. The goal of this paper was the detailed study of crystallization process, investigation of crystallization kinetics, such as sample concentration, composition, cooling speed and end-cooling temperature, application of pure seeding crystals with particular influence on the mass and composition of crystals.