Comparison of the Structure and the Stability of Single Enzyme Nanoparticles

Abstract

Single enzyme complex nanoparticles were synthesized following the method of Kim et al. The enzyme complex used was cellulosome that is a large spatial association of enzymes. Single enzyme nanoparticle of celluclast BG cellulase enzyme complex (SEN-CK) can degrade as great substrates as natural cellulose. It was proved that the stability of SEN-CK enzyme complex is significantly better than that of native enzyme complex (enzyme without pretreatment). Significant portion of the activity of SEN-CK is remaining at 80 °C for 1 hour time.